anr 94  (Tocris)

Journal: The FASEB Journal

Article Title: Distinctive immunoregulatory effects of adenosine on T cells of older humans

doi: 10.1096/fj.11-197046

Figure Lengend Snippet: Effects of adenosine and the A2A adenosine receptor antagonists SCH58261 (SCH) and ANR94 (ANR) on T-cell chemotaxis. T cells from 2 older men and women (n=4) and 2 younger matched control men and women were preincubated for 15 min and then continuously with 0.1, 0.3, or 1.0 μM adenosine, 10 μM EHNA, or 20 nM SCH58261 for 4 h of chemotaxis to 50 nM CCL21 or 100 nM S1P. T cells from 2 older men and 1 old woman (n=3) and 2 younger matched control men and 1 woman similarly were preincubated for 15 min and then continuously with 460 nM ANR94 for 4 h of chemotaxis to 50 nM CCL21 or 100 nM S1P. Bars represent means ± sd of each cluster of responses as a percentage of the control responses (100%). Mean CCL21 control chemotactic responses for adenosine, EHNA, and SCH series were 24.6 ± 7.1% of initial T cells for younger donors and 16.7 ± 3.8% of initial T cells for older donors (69% of young control group level). Mean CCL21 control chemotactic responses for ANR series were 31.8 ± 6.5% of initial T cells for younger donors and 24.7 ± 3.5% of initial T cells for older donors (68% of young control group level). Mean S1P control chemotactic responses for SCH and ANR series were 7.2 ± 1.1% of initial T cells for younger donors and 5.3 ± 0.6% of initial T cells for older donors (74% of young control group level). Symbols at tops of bars indicate statistically significant difference vs. corresponding control; symbols next to horizontal arrowheads indicate difference between bars of that set. +P < 0.05, *P < 0.01, #P < 0.01.

Article Snippet: The A 2A receptor antagonists ANR94 and SCH58261 and the A 2A receptor agonist CGS21680 hydrochloride (Tocris Biosciences, Ellisville, MO, USA), the A 3 receptor antagonist 2-phenyl-amino-N6-endo-norbornyladenine (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the ADA inhibitor erythro-9-(2-hydroxy-3-nonyl)-adenine hydrochloride (EHNA), adenosine, sphingosine 1-phosphate (S1P), and recrystallized bovine serum albumin (Sigma Chemicals, St. Louis, MO, USA) were obtained from the designated suppliers.

Techniques: Chemotaxis Assay, Control

Journal: The FASEB Journal

Article Title: Distinctive immunoregulatory effects of adenosine on T cells of older humans

doi: 10.1096/fj.11-197046

Figure Lengend Snippet: Effects of adenosine and the A2A adenosine receptor antagonists SCH58261 (SCH) and ANR94 (ANR) on T-cell chemotaxis. T cells from 2 older men and women (n=4) and 2 younger matched control men and women were preincubated for 15 min and then continuously with 0.1, 0.3, or 1.0 μM adenosine, 10 μM EHNA, or 20 nM SCH58261 for 4 h of chemotaxis to 50 nM CCL21 or 100 nM S1P. T cells from 2 older men and 1 old woman (n=3) and 2 younger matched control men and 1 woman similarly were preincubated for 15 min and then continuously with 460 nM ANR94 for 4 h of chemotaxis to 50 nM CCL21 or 100 nM S1P. Bars represent means ± sd of each cluster of responses as a percentage of the control responses (100%). Mean CCL21 control chemotactic responses for adenosine, EHNA, and SCH series were 24.6 ± 7.1% of initial T cells for younger donors and 16.7 ± 3.8% of initial T cells for older donors (69% of young control group level). Mean CCL21 control chemotactic responses for ANR series were 31.8 ± 6.5% of initial T cells for younger donors and 24.7 ± 3.5% of initial T cells for older donors (68% of young control group level). Mean S1P control chemotactic responses for SCH and ANR series were 7.2 ± 1.1% of initial T cells for younger donors and 5.3 ± 0.6% of initial T cells for older donors (74% of young control group level). Symbols at tops of bars indicate statistically significant difference vs. corresponding control; symbols next to horizontal arrowheads indicate difference between bars of that set. +P < 0.05, *P < 0.01, #P < 0.01.

Article Snippet: Chemicals The A 2A receptor antagonists ANR94 and SCH58261 and the A 2A receptor agonist CGS21680 hydrochloride (Tocris Biosciences, Ellisville, MO, USA), the A 3 receptor antagonist 2-phenyl-amino-N6-endo-norbornyladenine (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the ADA inhibitor erythro-9-(2-hydroxy-3-nonyl)-adenine hydrochloride (EHNA), adenosine, sphingosine 1-phosphate (S1P), and recrystallized bovine serum albumin (Sigma Chemicals, St. Louis, MO, USA) were obtained from the designated suppliers.

Techniques: Chemotaxis Assay, Control